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pcr 3 amplification  (Addgene inc)


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    Structured Review

    Addgene inc pcr 3 amplification
    Pcr 3 Amplification, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+3+amplification/pNIC28-Bsa4+(Plasmid+%2326103)/10__1016_slash_j__cellin__2026__100324-163-12-19
    Average 93 stars, based on 95 article reviews
    pcr 3 amplification - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Generated:

    Article Title: FINE-EM-seq: a rapid isothermal amplification method enabling comprehensive methylome profiling of zebrafish early embryos
    Article Snippet: .. Two 2-kb DNA spike-in controls, designated 2kb-2 and 2kb-3, were generated by PCR 3 amplification from the pNIC28-Bsa4 plasmid (Addgene, 26103) as described previously (Liu 4 et al., 2019) (sequence of primers listed in Table S6). .. To generate fully CpG-methylated spike-5 in control, the 2kb-2 amplicon was treated with M.SssI CpG methyltransferase (NEB, M0226), 6 according to a published protocol (Liu et al., 2019), while 2kb-3 remained untreated and used 7 as the unmethylated control.

    Polymerase Chain Reaction:

    Article Title: FINE-EM-seq: a rapid isothermal amplification method enabling comprehensive methylome profiling of zebrafish early embryos
    Article Snippet: .. Two 2-kb DNA spike-in controls, designated 2kb-2 and 2kb-3, were generated by PCR 3 amplification from the pNIC28-Bsa4 plasmid (Addgene, 26103) as described previously (Liu 4 et al., 2019) (sequence of primers listed in Table S6). .. To generate fully CpG-methylated spike-5 in control, the 2kb-2 amplicon was treated with M.SssI CpG methyltransferase (NEB, M0226), 6 according to a published protocol (Liu et al., 2019), while 2kb-3 remained untreated and used 7 as the unmethylated control.

    Amplification:

    Article Title: FINE-EM-seq: a rapid isothermal amplification method enabling comprehensive methylome profiling of zebrafish early embryos
    Article Snippet: .. Two 2-kb DNA spike-in controls, designated 2kb-2 and 2kb-3, were generated by PCR 3 amplification from the pNIC28-Bsa4 plasmid (Addgene, 26103) as described previously (Liu 4 et al., 2019) (sequence of primers listed in Table S6). .. To generate fully CpG-methylated spike-5 in control, the 2kb-2 amplicon was treated with M.SssI CpG methyltransferase (NEB, M0226), 6 according to a published protocol (Liu et al., 2019), while 2kb-3 remained untreated and used 7 as the unmethylated control.

    Plasmid Preparation:

    Article Title: FINE-EM-seq: a rapid isothermal amplification method enabling comprehensive methylome profiling of zebrafish early embryos
    Article Snippet: .. Two 2-kb DNA spike-in controls, designated 2kb-2 and 2kb-3, were generated by PCR 3 amplification from the pNIC28-Bsa4 plasmid (Addgene, 26103) as described previously (Liu 4 et al., 2019) (sequence of primers listed in Table S6). .. To generate fully CpG-methylated spike-5 in control, the 2kb-2 amplicon was treated with M.SssI CpG methyltransferase (NEB, M0226), 6 according to a published protocol (Liu et al., 2019), while 2kb-3 remained untreated and used 7 as the unmethylated control.

    Sequencing:

    Article Title: FINE-EM-seq: a rapid isothermal amplification method enabling comprehensive methylome profiling of zebrafish early embryos
    Article Snippet: .. Two 2-kb DNA spike-in controls, designated 2kb-2 and 2kb-3, were generated by PCR 3 amplification from the pNIC28-Bsa4 plasmid (Addgene, 26103) as described previously (Liu 4 et al., 2019) (sequence of primers listed in Table S6). .. To generate fully CpG-methylated spike-5 in control, the 2kb-2 amplicon was treated with M.SssI CpG methyltransferase (NEB, M0226), 6 according to a published protocol (Liu et al., 2019), while 2kb-3 remained untreated and used 7 as the unmethylated control.



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